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Characterization of Toxoplasma gondii 5' UTR with Encyclopedic TSS Information OAK
Yamagishi, J.; Watanabe, J.; Goo, Y. K.; Masatani, T.; Suzuki, Y.; Xuan, X.; 玄, 学南.
The 5′ UTR is widely involved in gene expression via post-transcriptional regulation. However, a detailed profile of the 5′ UTR for Toxoplasma gondii has not yet been demonstrated. To investigate the issue, we compared the predicted open reading frames (ORFs) and transcription start sites (TSSs) of T. gondii obtained by TSS-seq, a method that enables analysis of encyclopedic TSSs with next-generation sequencers. As a result, it was demonstrated that the mode length of the 5′ UTR is between 120 and 140 nucleotides (nts) when a subset of genes with predicted signal peptides was examined. However, when genes without the signal peptide were examined, the length was extended to approximately 600 nts. Because additional information on the predicted signal...
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3822
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